Coding

Part:BBa_K764006:Design

Designed by: Frederic Dudbridge   Group: iGEM12_Exeter   (2012-09-15)

WbbC


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Designing the primers with the prefix and suffix attached and correct conditions for PCR.


Source

PCR amplified from Escherichia coli Bl21(DE3), Escherichia coli, Accession Number: CP001509.

References

Jeong, H. et al. (2009) Genome sequences of Escherichia coli B strains REL606 and BL21(DE3), J. Mol. Biol. 394: 644-652.